This study presents a refined cytoarchitectonic parcellation of Broca’s region into four distinct subdivisions (44p, 44a, 45p, and 45a), revealing clear microstructural and functional differentiation. High-resolution 3D reconstructions and probabilistic maps capture interindividual variability and hemispheric asymmetries, while layer-specific segmentation enhances anatomical detail. Functional mapping and meta-analytic connectivity modelling suggest distinct roles of subregions in syntax, semantics, and action processing. All maps will be made available through the Julich-Brain Atlas on EBRAINS to support future neuroimaging and modeling research.